Sigma：明膠 來源于豬皮膚

屬性：

類型：Type A

質量水平：200

技術：cell culture | mammalian: suitable

說明：

應用：

This product is recommended for use as a cell culture substratum at 1-5 μg/cm2 or 0.5-50 μg/mL. The optimal concentration does depend on cell type as well as the application and research objectives.

Gelatin has been used in many applications. It has use in coating cell culture to improve attachment of cells, being added to PCR to stabilize Taq DNA, as a blocking reagent in Western blotting, ELISA, and immunochemistry, and as a component of media for species differentiation in bacteriology. As a biocompatible polymer, it has used as a delivery vehicle for release of active biomolecules and in generation of scaffolds for tissue engineering applications. In the pharmaceutical industry, geltan can be used as a suspending and encapsulating agent, among other applications.

組分：

明膠存在于膠原蛋白中，是一種具有高平均分子量的水溶性蛋白質的異質性混合物。 通過在水中將相關的皮膚、肌腱、韌帶、骨骼等進行煮沸來提取蛋白質。 A類明膠來自于酸固化組織。 B類明膠來自于石灰固化組織。

注意：

Dry gelatin, when stored in airtight containers at room temperature, will remain unchanged for many years. When heated at 100°C in the presence of air, it swells becomes soft and disintegrates to a carbonaceous mass with evolution of pyridine bases and ammonia.

制備說明：

This product is derived from porcine skin. Gelatin is soluble in hot than in cold water. It is practically insoluble in most organic solvents such as alcohol, chloroform, carbon disulfide, carbon tetrachloride, ether, benzene, acetone, and oils. The Bloom number, determined by the Bloom gelometer, is an indication of the strength of a gel formed from a solution of the known concentration. The Bloom number is proportional to the average molecular mass. Bloom numbers of porcine skin Gelatin vary from 90 to 300 g. This product has a gel strength of 80-120.

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主營產品:

試劑盒：ELISA試劑盒、免疫組化試劑盒、分子生物學試劑盒、細胞凋亡試劑盒

細胞培養(yǎng)：血清，培養(yǎng)基、胰酶、雙抗等

耗材：進口、國產細胞培養(yǎng)耗材、普通實驗儀器及耗材

生化試劑：進口原裝生化試劑、國產生化試劑

抗體：進口、國產原裝抗體、單克隆抗體、多克隆抗體、一抗、二抗等

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